Recombinant Human GPC1 protein, His-tagged

• Cat.No.: GPC1-152H
• Product Overview: Recombinant Human GPC1 protein(P35052-1)(Asp24-Ser530), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Asp24-Ser530
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Glypican 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Glypican 1 Antibody, hFc Tag with the EC50 of 21.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 57.1 kDa. Due to glycosylation, the protein migrates to 70-135 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GPC1 glypican 1 [ Homo sapiens ]
• Official Symbol: GPC1
• Synonyms: GPC1; glypican 1; glypican-1; glypican; glypican proteoglycan 1; FLJ38078
• Gene ID: 2817
• mRNA Refseq: NM_002081
• Protein Refseq: NP_002072
• MIM: 600395
• UniProt ID: P35052