Recombinant Human GP6 protein, His-tagged

• Cat.No.: GP6-5623H
• Product Overview: Recombinant Human GP6 protein(Q9HCN6-1)(Gln21-Lys267), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Gln21-Lys267
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GPVI, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GP6 glycoprotein VI (platelet) [ Homo sapiens ]
• Official Symbol: GP6
• Synonyms: GP6; glycoprotein VI (platelet); platelet glycoprotein VI; GPVI; glycoprotein 6; platelet collagen receptor; GPIV; BDPLT11; MGC138168
• Gene ID: 51206
• mRNA Refseq: NM_001083899
• Protein Refseq: NP_001077368
• MIM: 605546
• UniProt ID: Q9HCN6