Active Recombinant Mouse Glycoprotein 6 (platelet), His-tagged

• Cat.No.: Gp6-5622M
• Product Overview: RecombinantMouse Gp6 protein was expressed in Murine myeloma cell line. Met1-Lys265,with a C-terminal 6-His tag.

Specification

• Cat. No.: Gp6-5622M
• Description: Gp6 is a63 kDa platelet/megakaryocyte-specific type I transmembrane glycoprotein ofthe immunoglobulin superfamily that is an important collagen receptor andinitiator of platelet activation, aggregation and thrombus generation. Gp6 isalso a secondary receptor required for platelet spreading on laminin. Mouse Gp6contains a 21 amino acid (aa) signal sequence, a 244 aa extracellular domain (ECD)that has two C-type Ig-like domains followed by a mucin-like, presumablyO-glycosylated Ser-Thr-rich region, a 21 aa transmembrane (TM) domain and a27 aa cytoplasmic tail. Mouse Gp6 ECD shares 70%, 85%, 69% and 66% aaidentity with human, rat, equine and canine Gp6 ECD, respectively.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Gly24
• MolecularWeight: 27.8 kDa (monomer)
• Activity: Measuredby its binding ability in a functional ELISA. When cross-linked with 10 μg/mLof Mouse polyHistidine Monoclonal Antibody. Recombinant Mouse Gp6 binds toCollagen I (coated at 10 μg/mL, 100 μL/well) with an apparent KD < 90 nM.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 45-60kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 400μg/mL in PBS.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
• Protein length: 1-265 a.a.

Gene Information

• Gene Name: Gp6 glycoprotein 6 (platelet) [ Mus musculus ]
• Official Symbol: Gp6
• Synonyms: Gp6; glycoprotein6 (platelet); Gpvi; Gm469; 9830166G18Rik; platelet glycoprotein VI;glycoprotein 5
• Gene ID: 243816
• mRNA Refseq: NM_001163014
• Protein Refseq: NP_001156486
• UniProt ID: P0C191
• Chromosome Location: 7
• Pathway: Cell surfaceinteractions at the vascular wall; ECM-receptor interaction; GPVI-mediatedactivation cascade; Hemostasis; Platelet Adhesion to exposed collagen; Plateletactivation
• Function: collagen binding; receptoractivity