Recombinant Human GNAO1, GST-tagged

• Cat.No.: GNAO1-13349H
• Product Overview: Recombinant Human GNAO1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
• Protein length: 1-302a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: GNAO1 guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O [ Homo sapiens ]
• Official Symbol: GNAO1
• Synonyms: GNAO1; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide-binding protein G(o) subunit alpha; G ALPHA o; guanine nucleotide binding protein, alpha activating polypeptide O; GNAO; G-ALPHA-o; DKFZp686O0962
• Gene ID: 2775
• mRNA Refseq: NM_020988
• Protein Refseq: NP_066268
• MIM: 139311
• UniProt ID: P09471
• Chromosome Location: 16q13
• Pathway: Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cholinergic synapse, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; G Protein Signaling Pathways, organism-specific biosystem
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled serotonin receptor binding; GTP binding; GTPase activity; guanyl nucleotide binding; metal ion binding; mu-type opioid receptor binding; nucleotide binding; phosphatidylinositol phospholipase