Active Recombinant Human GLO1 Protein, Met & His-tagged

• Cat.No.: GLO1-574H
• Product Overview: Recombinant Human GLO1 (Accession # NP_006699) Ala2-Met184, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-HCl and DTT.
• Bio-activity: Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione. The specific activity is >100 nmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human GLO1, Met & His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 25 kDa, reducing conditions.
• Protein length: Ala2-Met184
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: GLO1 glyoxalase I [ Homo sapiens ]
• Official Symbol: GLO1
• Synonyms: GLO1; glyoxalase I; lactoylglutathione lyase; GLOD1; glyoxalase domain containing 1; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; S-D-lactoylglutathione methylglyoxal lyase; GLYI
• Gene ID: 2739
• mRNA Refseq: NM_006708
• Protein Refseq: NP_006699
• MIM: 138750
• UniProt ID: Q04760
• Chromosome Location: 6p21.3-p21.1