Recombinant Human GLUD1 Protein, His-tagged

• Cat.No.: GLUD1-387H
• Product Overview: Recombinant Human GLUD1(Asp334~Thr556) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Asp334~Thr556
• Description: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 28.7kDa
• Identity: Reconstitute in PBS or others
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: GLUD1 glutamate dehydrogenase 1 [ Homo sapiens ]
• Official Symbol: GLUD1
• Synonyms: GLUD1; glutamate dehydrogenase 1; GLUD; glutamate dehydrogenase 1, mitochondrial; GDH; GDH 1; glutamate dehydrogenase (NAD(P)+); GDH1; MGC132003
• Gene ID: 2746
• mRNA Refseq: NM_005271
• Protein Refseq: NP_005262
• MIM: 138130
• UniProt ID: P00367