Recombinant Human Glucose-6-Phosphate 1-Dehydrogenase

• Cat.No.: G6PD-192H
• Product Overview: Recombinant G6PD protein was expressed inE.coliand purified by conventional chromatography techniques. MW = 55.7kDa (491 aa).

Specification

• Cat. No.: G6PD-192H
• Description: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-δ-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
• Source: E.coli.
• Amino Acid Sequence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWV DSITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E.
• Form: Liquid. In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT,10% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Biological activity: Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C.
• Activity Assay: 1.Prepare a 3ml reaction cocktail into a suitable container: The final concentrations are 50mM gylcylglycine, 2mM D-glucose 6-phosphate, 0.67mM beta-NADP, 10mM MgCl2.2.Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3.Add 4ug of recombinant G6PD into reaction cocktail and mix immediately. 4.Record the increase in A340nm for 5 minutes.
• Storagee: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Tag: Non

Gene Information

• Synonyms: G6PD; Glucose-6-phosphate 1-dehydrogenase
• Gene ID: 25399
• mRNA Refseq: NM_000402
• Protein Refseq: NP_000393
• MIM: 305900
• UniProt ID: P11413
• Chromosome Location: Xq28
• Function: glucose-6-phosphate dehydrogenase activity; oxidoreductase activity; binding