Recombinant Human GLP1R Transmembrane protein, His&Flag-tagged(Nanodisc)

• Cat.No.: GLP1R-1934H
• Product Overview: Recombinant Human GLP1R Transmembrane protein(P43220) (Arg24-Phe257 & Glu262-Lys437,thermostabilizing mutations) was expressed in HEK293, with a polyhistidine tag and Flag tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: C-His&Flag
• ProteinLength: Arg24-Phe257 & Glu262-Lys437, thermostabilizing mutations
• Form: Supplied as sterile 50 mM HEPES, 150 mM NaCl, pH 7.5.
• Bio-activity: Immobilized Recombinant Human GLP1R-Nanodisc Protein at 5 μg/mL (100 μL/well) can bind Anti-GLP1R Monoclonal Antibody, Human IgG1, the EC50 is 2-8 ng/mL.
• Molecular Mass: It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -70°C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GLP1R glucagon-like peptide 1 receptor [ Homo sapiens ]
• Official Symbol: GLP1R
• Synonyms: GLP1R; glucagon-like peptide 1 receptor; GLP-1R; GLP-1-R; GLP-1 receptor; MGC138331
• Gene ID: 2740
• mRNA Refseq: NM_002062
• Protein Refseq: NP_002053
• MIM: 138032
• UniProt ID: P43220