Recombinant Human GLP1R protein, mFc-tagged

• Cat.No.: GLP1R-584H
• Product Overview: Recombinant Human GLP1R protein(P43220)(Arg24-Glu139) is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• ProteinLength: Arg24-Glu139
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GLP-1R, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GLP-1R Antibody, hFc Tag with the EC50 of 17.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.20 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GLP1R glucagon-like peptide 1 receptor [ Homo sapiens ]
• Official Symbol: GLP1R
• Synonyms: GLP1R; glucagon-like peptide 1 receptor; GLP-1R; GLP-1-R; GLP-1 receptor; MGC138331
• Gene ID: 2740
• mRNA Refseq: NM_002062
• Protein Refseq: NP_002053
• MIM: 138032
• UniProt ID: P43220