Recombinant Human GIP protein, His-tagged

• Cat.No.: GIP-7855H
• Product Overview: Recombinant Human GIP protein(P09681)(Glu22-Gln93), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Glu22-Gln93
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GIP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GIP gastric inhibitory polypeptide [ Homo sapiens ]
• Official Symbol: GIP
• Synonyms: GIP; gastric inhibitory polypeptide; glucose dependent insulinotropic polypeptide; glucose-dependent insulinotropic polypeptide
• Gene ID: 2695
• mRNA Refseq: NM_004123
• Protein Refseq: NP_004114
• MIM: 137240
• UniProt ID: P09681