Recombinant Human ghrelin/obestatin prepropeptide Protein, His&Flag tagged

• Cat.No.: GHRL-220H
• Product Overview: Recombinant human GHRL protein (Q9UBU3) (Gly 24-Lys 117) with both His, Flag tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 24-117aa
• Description: This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.
• Tag: C-His&Flag
• Molecular Mass: 13.2 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: GHRL ghrelin/obestatin prepropeptide [ Homo sapiens (human) ]
• Official Symbol: GHRL
• Synonyms: GHRL; ghrelin/obestatin prepropeptide; ghrelin, growth hormone secretagogue receptor ligand; appetite-regulating hormone; ghrelin; MTLRP; obestatin; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide
• Gene ID: 51738
• mRNA Refseq: NM_001134941
• Protein Refseq: NP_001128413
• MIM: 605353
• UniProt ID: Q9UBU3