Recombinant Human GGT5 protein, His & T7-tagged

• Cat.No.: GGT5-1145H
• Product Overview: Recombinant Human GGT5 aa. (Thr388~Tyr586 (Accession # P36269) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.5kDa.
• Protein length: Thr388~Tyr586
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GGT5 gamma-glutamyltransferase 5 [ Homo sapiens (human) ]
• Official Symbol: GGT5
• Synonyms: GGT5; gamma-glutamyltransferase 5; GGL; GGT 5; GGTLA1; GGT-REL; gamma-glutamyl cleaving enzyme; gamma-glutamyl transpeptidase-related enzyme; gamma-glutamyl transpeptidase-related protein; gamma-glutamyltransferase-like activity 1; gamma-glutamyltranspeptidase 5; glutathione hydrolase 5; leukotriene-C4 hydrolase
• Gene ID: 2687
• mRNA Refseq: NM_001099781.2
• Protein Refseq: NP_001093251.1
• UniProt ID: P36269