Recombinant Human GDF5 Protein (Ala382-Arg501), His tagged

• Cat.No.: GDF5-2449H
• Product Overview: Recombinant human GDF5 (Ala382-Arg501) protein fused with His tag was expressed in E. coli.

Specification

• Description: Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 15.1 kDa
• Protein length: Ala382-Arg501
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: GDF5 growth differentiation factor 5 [ Homo sapiens (human) ]
• Official Symbol: GDF5
• Synonyms: GDF5; growth differentiation factor 5; growth/differentiation factor 5; BMP14; cartilage derived morphogenetic protein 1; CDMP1; GDF-5; CDMP-1; radotermin; cartilage-derived morphogenetic protein-1; OS5; LAP4; SYNS2
• Gene ID: 8200
• mRNA Refseq: NM_000557
• Protein Refseq: NP_000548
• MIM: 601146
• UniProt ID: P43026

Reviews

06/26/2022

Their dedicated team of experts is prompt, knowledgeable, and readily available to tackle any hurdles or concerns I may encounter.

03/20/2020

Used in protein electron microscopy structure analysis.Great.

08/31/2019

By harnessing the power of the GDF5 protein, I can harness its outstanding quality and tap into its potential to obtain dependable and meaningful findings.

Q&As

How does GDF5 contribute to cartilage repair?

GDF5 has been found to stimulate the proliferation and differentiation of chondrocytes, the cells responsible for cartilage formation and maintenance.

What preclinical evidence supports the use of GDF5 in orthopedics?

Preclinical studies have demonstrated GDF5's ability to promote bone and cartilage regeneration in animal models, providing a foundation for further clinical exploration.

How does GDF5 differ from other growth factors in terms of clinical applications?

GDF5's specificity for bone and cartilage development distinguishes it from other growth factors, making it a targeted option for orthopedic interventions.

Can GDF5 be used in combination with other therapies for enhanced effects?

Some studies suggest that combining GDF5 with other growth factors or therapeutic approaches may result in synergistic effects, improving overall outcomes.

Is GDF5 only relevant for bone and cartilage conditions, or are there other potential applications?

While its primary focus is on musculoskeletal conditions, GDF5's role in cellular differentiation opens avenues for exploring its applications in broader regenerative medicine contexts.