Recombinant Human GCOM1 293 Cell Lysate

• Cat.No.: GCOM1-5977HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for GRINL1A complex locus (GCOM1), transcript variant 12 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GCOM1 GRINL1A complex locus 1 [ Homo sapiens ]
• Official Symbol: GCOM1
• Synonyms: GCOM1; GRINL1A complex locus 1; GRINL1A combined protein; FLJ30973; GRINL1A; MYZAP POLR2M; MYZAP POLR2M readthrough; MYZAP-POLR2M protein; MYZAP-POLR2M readthrough; GRINL1A combined protein Gcom12; GRINL1A complex locus protein 1; NMDAR1 subunit-interacting protein; myocardial zonula adherens protein; glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A combined protein; gcom; Gcom2; MYZAP; MYZAP-POLR2M; MGC126694; MGC138353
• Gene ID: 145781
• mRNA Refseq: NM_001018100
• Protein Refseq: NP_001018110
• MIM: 614071
• Chromosome Location: 15q21.3