Recombinant Human GCKR protein, His-tagged

• Cat.No.: GCKR-6859H
• Product Overview: Recombinant Human GCKR aa. (Gln91~Ala286 (Accession # Q14397)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.3kDa.
• Protein length: Gln91~Ala286
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: GCKR glucokinase regulator [ Homo sapiens (human) ]
• Official Symbol: GCKR
• Synonyms: GKRP; FGQTL5; glucokinase (hexokinase 4) regulator; hexokinase 4 regulator; glucokinase regulatory protein
• Gene ID: 2646
• mRNA Refseq: NM_001486.3
• Protein Refseq: NP_001477.2
• UniProt ID: Q14397