Recombinant Human GCHFR, His-tagged

• Cat.No.: GCHFR-245H
• Product Overview: GCHFR, 1-84aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-84aa
• Description: GTP cyclohydrolase I feedback regulatory protein(GCHFR) binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. Recombinant human GCHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 40% glycerol, 1mM DTT
• Molecular Mass: 12.1 kDa (107aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPYLLIS TQIRMEVGPT MVGDEQSDPE LMQHLGASKR RALGNNFYEY YVDDPPRIVL DKLERRGFRV LSMTGVGQTL VWCLHKE
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GCHFR GTP cyclohydrolase I feedback regulator [ Homo sapiens ]
• Official Symbol: GCHFR
• Synonyms: GCHFR; GTP cyclohydrolase I feedback regulator; GTP cyclohydrolase I feedback regulatory protein; GTP cyclohydrolase 1 feedback regulatory protein; GFRP; HsT16933; P35; MGC138467; MGC138469
• Gene ID: 2644
• mRNA Refseq: NM_005258
• Protein Refseq: NP_005249
• MIM: 602437
• UniProt ID: P30047
• Chromosome Location: 15q15
• Pathway: Metabolism, organism-specific biosystem; Metabolism of nitric oxide, organism-specific biosystem; Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation, organism-specific biosystem; eNOS activation and regulation, organism-specific biosystem
• Function: GTP cyclohydrolase I binding; GTP-dependent protein binding; amino acid binding; enzyme inhibitor activity; protein binding