Recombinant Human GARS, His-tagged

• Cat.No.: GARS-263H
• Product Overview: GARS, 43-289aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 43-289aa
• Description: GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 30.0 kDa (270aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSPISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GARS glycyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: GARS
• Synonyms: GARS; glycyl-tRNA synthetase; Charcot Marie Tooth neuropathy 2D , CMT2D; glycine--tRNA ligase; DSMAV; glycine tRNA ligase; GlyRS; SMAD1; AP-4-A synthetase; Charcot-Marie-Tooth neuropathy 2D; diadenosine tetraphosphate synthetase; Charcot-Marie-Tooth neuropathy, neuronal type, D; HMN5; CMT2D
• Gene ID: 2617
• mRNA Refseq: NM_002047
• Protein Refseq: NP_002038
• MIM: 600287
• UniProt ID: P41250
• Chromosome Location: 7p15
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Mitochondrial tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; glycine-tRNA ligase activity; glycine-tRNA ligase activity; ligase activity; nucleotide binding; protein dimerization activity