Recombinant Human GAPDH Protein, His-tagged

• Cat.No.: GAPDH-045H
• Product Overview: Recombinant Human GAPDH Protein with His tag was expressed in E. coli.

Specification

• Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~36 kDa
• AA Sequence: MGKVKVGVNGFGRIGRLVTRAAFNS GKVDIVAINDPFIDLNYMVYMFQYD STHGKFHGTVKAENGKLVINGNPIT IFQERDPSKIKWGDAGAEYVVESTG VFTTMEKAGAHLQGGAKRVIISAPS ADAPMFVMGVNHEKYDNSLKIISNA SCTTNCLAPLAKVIHDNFGIVEGLM TTVHAITATQKTVDGPSGKLWRDGR GALQNIIPASTGAAKAVGKVIPELN GKLTGMAFRVPTANVSVVDLTCRLE KPAKYDDIKKVVKQASEGPLKGILG YTEHQVVSSDFNSDTHSSTFDAGAG IALNDHFVKLISWYDNEFGYSNRVV DLMAHMASKE
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, pH7.4

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685
• Gene ID: 2597
• mRNA Refseq: NM_001256799
• Protein Refseq: NP_001243728
• MIM: 138400
• UniProt ID: P04406

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.