Recombinant Human GAPDH

• Cat.No.: GAPDH-26354TH
• Product Overview: Recombinant full length Human GAPDH, amino acids 1-335, MWt 35.9kDa.

Specification

• Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
• Source: E. coli
• Biological activity: Specific activity : 35.7 U/mgOne unit of activity of GAPDH is defined as the amount of enzyme required to convert 1μmole of NAD to NADH per min under standard assay conditions.
• Form: Lyophilised:Reconstitute with HEPES or Tris buffer. Add 10% glycerol for longer term storage.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM HEPES, pH 7.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• Tag: Non
• Protein length: 1-335 a.a.
• Full Length: Full L.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD
• Gene ID: 2597
• mRNA Refseq: NM_002046
• Protein Refseq: NP_002037
• MIM: 138400
• Uniprot ID: P04406
• Chromosome Location: 12p13.31
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>
• Function: NAD binding; NADP binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; oxidoreductase activity