Recombinant Human GAD2 Protein, His-tagged

• Cat.No.: GAD2-1556H
• Product Overview: Recombinant Human GAD2 Protein (Met188-Ser374) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met188-Ser374
• Description: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.9 kDa
  Accurate Molecular Mass: 20 kDa
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GAD2 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) [ Homo sapiens (human) ]
• Official Symbol: GAD2
• Synonyms: GAD2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2; GAD65; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; MGC161605; MGC161607
• Gene ID: 2572
• mRNA Refseq: NM_000818
• Protein Refseq: NP_000809
• MIM: 138275
• UniProt ID: Q05329