Recombinant Human G antigen 12F, His-tagged

• Cat.No.: GAGE12F-304H
• Product Overview: GAGE12F, 1-117 aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-117 a.a.
• Description: GAGE12F belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE12F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
• Molecular Mass: 15.4 kDa (140aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSWRGRS TYYWPRPRRY VQPPEMIGPM RPEQFSDEVE PATPEEGEPA TQRQDPAAAQ EGEDEGASAG QGPKPEAHSQ EQGHPQTGCE CEDGPDGQEM DPPNPEEVKT PEEGEKQSQC
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by BCA assay)

Gene Information

• Gene Name: GAGE12F?G antigen 12F [?Homo sapiens?(human) ]
• Official Symbol: GAGE12F
• Synonyms: GAGE12F; G antigen 12F; GAGE-12F; g antigen 12F/G/I
• Gene ID: 100008586
• mRNA Refseq: NM_001098405
• Protein Refseq: NP_001091875
• MIM: 300730
• UniProt ID: P0CL80
• Chromosome Location: Xp11.23