Recombinant Human FST protein(Met1-Trp344), hFc-tagged

• Cat.No.: FST-3848H
• Product Overview: Recombinant Human FST (NP_037541.1) (Met 1-Trp 344) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: 1-344 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human INHBA-his in a functional ELISA. 2. Measured by its ability to bind mouse INHBA-his in a functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 10 ng/ml Recombinant Human ctivin A.
• Molecular Mass: The secreted recombinant human FST/Fc is a disulfide-linked homodimer. The reduced monomer consists of 556 amino acids and has a predicted molecular mass of 61.7 kDa. As a result of glycosylation, rh FST/Fc monomer migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FST follistatin [ Homo sapiens ]
• Official Symbol: FST
• Synonyms: FST; follistatin; FS; activin-binding protein; follistatin isoform FST317
• Gene ID: 10468
• mRNA Refseq: NM_006350
• Protein Refseq: NP_006341
• MIM: 136470
• UniProt ID: P19883