Recombinant Human FOXP3 293 Cell Lysate

• Cat.No.: FOXP3-6145HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for forkhead box P3 (FOXP3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FOXP3 forkhead box P3 [ Homo sapiens ]
• Official Symbol: FOXP3
• Synonyms: FOXP3; forkhead box P3; immune dysregulation, polyendocrinopathy, enteropathy, X linked , IPEX; forkhead box protein P3; AIID; DIETER; JM2; PIDX; SCURFIN; XPID; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; IPEX; MGC141961; MGC141963
• Gene ID: 50943
• mRNA Refseq: NM_001114377
• Protein Refseq: NP_001107849
• MIM: 300292
• UniProt ID: Q9BZS1
• Chromosome Location: Xp11.23
• Pathway: Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; IL2 signaling events mediated by STAT5, organism-specific biosystem
• Function: DNA binding, bending; NF-kappaB binding; NFAT protein binding; chromatin binding; double-stranded DNA binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription corepressor activity; zinc ion binding