Recombinant Human FOXM1 293 Cell Lysate

• Cat.No.: FOXM1-6152HCL

Specification

• Description: Antigen standard for forkhead box M1 (FOXM1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: FOXM1 forkhead box M1 [ Homo sapiens ]
• Official Symbol: FOXM1
• Synonyms: FOXM1; forkhead box M1; FKHL16; forkhead box protein M1; HFH 11; HNF 3; INS 1; M phase phosphoprotein 2; MPHOSPH2; MPP2; TGT3; trident; M-phase phosphoprotein 2; HNF-3/fork-head homolog 11; transcription factor Trident; MPM-2 reactive phosphoprotein 2; forkhead-related protein FKHL16; winged-helix factor from INS-1 cells; Forkhead, drosophila, homolog-like 16; hepatocyte nuclear factor 3 forkhead homolog 11; HFH11; HNF-3; INS-1; MPP-2; PIG29; FOXM1B; HFH-11; TRIDENT
• Gene ID: 2305
• mRNA Refseq: NM_001243088
• Protein Refseq: NP_973732
• MIM: 602341
• UniProt ID: Q08050
• Chromosome Location: 12p13
• Pathway: FOXM1 transcription factor network, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem
• Function: DNA binding; DNA binding; DNA binding, bending; double-stranded DNA binding; protein binding; protein domain specific binding; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding