Recombinant Human FOS, His-tagged

• Cat.No.: FOS-1026H

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: C-Fos is a cellular proto-oncogene belonging to the immediate early gene family of transcription factors. c-Fos has a leucine-zipper DNA binding domain, and a transactivation domain at the C-terminus. Transcription of c-Fos is upregulated in response to many extracellular signals, e.g. growth factors. Additionally, phosphorylation by MAPK, PKA, PKC or cdc2 alters the activity and stability of c-Fos. Members of the Fos family dimerise with C-Jun to form the AP-1 transcription factor, which upregulates transcription of a diverse range of genes involved in everything from proliferation and differentiation to defense against invasion and cell damage. The combinatorial diversity of AP-1 proteins and other interacting factors appears to influence how specific cell types respond to a stimulus. His tagged recombinant c-fos was expressed in a Baculovirus system and purified by an affinity column in combination with FPLC chromatography.
• Form: Liquid. Supplied in 20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
• Activity: 100ng are sufficient for a protein-protein interaction assay.
• Application: C-fos has been applied in protein-DNA and protein-protein interaction assays.
• Purity: >90% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: FOS FBJ murine osteosarcoma viral oncogene homolog [ Homo sapiens ]
• Synonyms: FOS; FBJ murine osteosarcoma viral oncogene homolog; AP-1; C-FOS; G0S7; c-fos; Proto-oncogene protein c-fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); activator protein 1; cellular oncogene c-fos; v-fos FBJ murine osteosarcoma viral oncogene homolog
• Gene ID: 2353
• mRNA Refseq: NM_005252
• Protein Refseq: NP_005243
• MIM: 164810
• UniProt ID: P01100
• Chromosome Location: 14q24.3
• Pathway: B cell receptor signaling pathway; Colorectal cancer; MAPK signaling pathway; Pathways in cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway
• Function: R-SMAD binding; double-stranded DNA binding; promoter binding; protein heterodimerization activity; specific RNA polymerase II transcription factor activity; transcription factor activity