Recombinant Human FNDC5 protein, His-tagged

• Cat.No.: FNDC5-542H
• Product Overview: Recombinant Human FNDC5 (Q8NAU1) aa. (Leu2~Ile137) fused with His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.6kDa; Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu2~Ile137
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Characteristic: Isoelectric point is 6.2.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: FNDC5 fibronectin type III domain containing 5 [ Homo sapiens (human) ]
• Official Symbol: FNDC5
• Synonyms: FNDC5; fibronectin type III domain containing 5; FRCP2; irisin; fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2
• Gene ID: 252995
• mRNA Refseq: NM_001171940.1
• Protein Refseq: NP_001165411.2
• UniProt ID: Q8NAU1