Recombinant Human Fms-Related Tyrosine Kinase 3

• Cat.No.: FLT3-1226H
• Product Overview: Recombinant humanFLT3 Ligand contains 155 amino acids and a 14 amino acid Histidine-based tagfor a total length of 169 amino acids.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: Non
• Description: The predicted molecular weight of therecombinant FLT3 Ligand is 19.9 kDa, but the recombinant protein migrates astwo bands with an apparent MW 20 and 22 kDa in SDS-PAGE as a result ofglycosylation.
• MolecularWeight: 20 and 22 kDa
• Form: Lyophilized from a0.2 µm sterile filtered solution in 10 mM acetic acid, pH 5.0.
• Purity: > 98% asdetermined by SDS-PAGE analysis. Purified product carries no pyrogenic orpro-inflammatory contaminants, as assayed with monocyte activation test usingHuman 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1betainduction.
• Endotoxin Level: < 0.005 ng/µg ofFLT3 Ligand
• Biological Activity: Determined by CellProliferation Assay for the dose-dependent effects of serial dilutions ofrecombinant human FLT3 Ligand on AML5 cells. The ED50 is typicallyin the range of 0.9-1.4 ng/ml.
• Specific Activity: 1.11 x 106units/mg
• Reconstitution: Centrifuge vialprior to opening. Add sterile PBS to the vial to fully solubilize theprotein to a concentration of 10 µg/ml.
• Storage: Store lyophilizedprotein at -20°C and reconstituted protein in working aliquots at -20°C witha carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Pleasenote that the addition of any carrier protein into this product may produceunwanted endotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homosapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosinekinase 3; FLK2; STK1; CD135; CD135 antigen; fetal liver kinase 2; FL cytokinereceptor; tal liver kinase 2; stem cell tyrosine kinase 1; FLT3 receptortyrosine kinase; tyrosine-protein kinase receptor FLT3; growth factorreceptor tyrosine kinase type III; C 2.7.10.1; OTTHUMP00000042340; fetalliver kinase 2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12
• Pathway: Acute myeloidleukemia; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage;Pathways in cancer
• Function: ATP binding; nucleotidebinding; osphoinositide 3-kinase binding; protein binding; receptor activity;transferase activity; vascular endothelial growth factor receptor activity