Recombinant Human FLT3, GST-His

• Cat.No.: FLT3-688H
• Product Overview: Recombinant human Flt-3 fused at the N-terminus to a GST-His6 thrombin cleavage site sequence was expressed inS. frugiperdainsect cells using a baculovirus expression system. MW = 82076 Da.

Specification

• Species: Human
• Source: S.frugiperda
• Tag: GST
• Description: Flt-3 belongs to the class III receptor tyrosine kinase family that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in vitro in cooperation with other cytokines. Flt-3 is also expressed in the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells.
• Form: Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20% glycerol, pH 8.0. Please refer to vial label for lot-specific concentration.
• Unit Definition: One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Glu,Tyr)4:1 per min at 30°C, using variable concentrations of ATP (0.1-12.6 µM).
• Specific Activity: ≥28 U/mg protein.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Synonyms: FLT3; fms-related tyrosine kinase 3; FLK2; STK1; CD135; CD135 antigen; fetal liver kinase 2; FL cytokine receptor; tal liver kinase 2; stem cell tyrosine kinase 1; FLT3 receptor tyrosine kinase; tyrosine-protein kinase receptor FLT3; growth factor receptor tyrosine kinase type III; C 2.7.10.1; OTTHUMP00000042340; fetal liver kinase 2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12
• Pathway: ute myeloid leukemia; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Pathways in cancer
• Function: ATP binding; nucleotide binding; osphoinositide 3-kinase binding; protein binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity