Recombinant Human Fms-Related Tyrosine Kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)

• Cat.No.: FLT1-1221H
• Product Overview: Recombinant humansoluble FLT1 is the naturally occurring form and was cloned from total RNA ofhuman umbilical vein endothelial cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: The recombinant mature soluble FLT1 is aglycosylated monomeric protein consisting of the first 6 extracellulardomains (Met1-His688) containing the unique 31 amino acid residues at theC-terminus.
• Form: Lyophilizedwithout additives.
• Molecular Weight: 96 kDa
• Purity: > 90% asdetermined by SDS-PAGE and visualized by silver stain
• Endotoxin Level: < 0.1 ng/µg of FLT1
• Biological Activity: Determined by itsability to abolish the binding of iodinated FLT1 to solid surfaces or cellsurface receptors. The ED50 for this effect is typically 10 ng/ml. The usablerange is 5-50 ng/ml.
• Reconstitution: Centrifugevial prior to opening. Soluble in water and most aqueous buffers. Add PBS tothe vial to fully solubilize the protein to a concentration not lower than100 ng/ml.
• Storage: Lyophilized proteinis stable for 6 months at -20°C to -80°C. Store reconstituted protein inworking aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: FLT1 fms-related tyrosine kinase 1(vascular endothelial growth factor/vascular permeability factor receptor) [ Homosapiens ]
• Official Symbol: FLT1
• Synonyms: FLT1; fms-related tyrosinekinase 1 (vascular endothelial growth factor/vascular permeability factorreceptor); FLT; VEGFR1; FRT; vascular endothelial growth factor receptor 1;EC 2.7.10.1; VEGFR-1; Vascular permeability factor receptor; Flt-1;Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-liketyrosine kinase 1
• Gene ID: 2321
• mRNA Refseq: NM_001159920
• Protein Refseq: NP_001153392
• MIM: 165070
• UniProt ID: P17948
• Chromosome Location: 13q12
• Pathway: Cytokine-cytokinereceptor interaction; Endocytosis; Focal Adhesion; Glypican 1 network;HIF-2-alpha transcription factor network; Id Signaling Pathway; Neurophilininteractions with VEGF and VEGFR; Rheumatoid arthritis; S1P3 pathway; SignalTransduction; Signaling by VEGF; Signaling events mediated by VEGFR1 andVEGFR2; VEGF binds to VEGFR leading to receptor dimerization; VEGFligand-receptor interactions; VEGFR1 specific signals
• Function: ATP binding; growth factorbinding; identical protein binding; nucleotide binding; receptor activity;transferase activity; vascular endothelial growth factor receptor activity