Recombinant Human FLG protein, His-tagged

• Cat.No.: FLG-1229H
• Product Overview: Recombinant Human FLG(Ser3905~Tyr4031) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser3905~Tyr4031
• Description: The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 25kDa
• AA Sequence: SHRDTA SHVRSSPVQS DSSTAKEHGH FSSLSQDSAY HSGIQSRGSP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FLG filaggrin [ Homo sapiens ]
• Official Symbol: FLG
• Synonyms: FLG; filaggrin; epidermal filaggrin; ATOD2
• Gene ID: 2312
• mRNA Refseq: NM_002016
• Protein Refseq: NP_002007
• MIM: 135940
• UniProt ID: P20930
• Chromosome Location: 1q21.3
• Function: calcium ion binding; nucleoside-triphosphatase activity; structural molecule activity