Recombinant Human FLAD1 293 Cell Lysate

• Cat.No.: FLAD1-6197HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FLAD1 FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: FLAD1
• Synonyms: FLAD1; FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae); Fad1, flavin adenine dinucleotide synthetase, homolog (yeast); FAD synthase; FAD1; PP591; FAD synthetase; FAD-synthetase; FAD pyrophosphorylase; FMN adenylyltransferase; flavin adenine dinucleotide synthase; flavin adenine dinucleotide synthetase; Fad1, flavin adenine dinucleotide synthetase, homolog; FADS; MGC31803; MGC40255; RP11-307C12.7
• Gene ID: 80308
• mRNA Refseq: NM_001184891
• Protein Refseq: NP_001171820
• MIM: 610595
• UniProt ID: Q8NFF5
• Chromosome Location: 1q22
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; Riboflavin metabolism, organism-specific biosystem; Riboflavin metabolism, conserved biosystem; Selenium Pathway, organism-specific biosystem
• Function: ATP binding; FMN adenylyltransferase activity; catalytic activity; nucleotide binding; nucleotidyltransferase activity; transferase activity