Recombinant Human FK506 Binding Protein 1A, 12kDa, His-tagged

• Cat.No.: FKBP1A-503H
• Product Overview: Recombinant human FKBP12 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW=14.1kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1, 4, 5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE.
• From: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 1mM DTT, 10% glycerol.
• Purity: >95% by SDS–PAGE.
• Concentration: 1mg/ml (determined by Bradford assay).
• Endotoxin Level: <1.0 EU per 1μg of protein (determined by LAL method ).
• Biological activity: Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.
• Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant FKBP1a/FKBP12 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• Storage: Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: FKBP1A FK506 binding protein 1A, 12kDa [ Homo sapiens ]
• Synonyms: FKBP1A; FK506 binding protein 1A, 12kDa; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP12C; FKBP1A; FK506-binding protein 1A (12kD); Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; EC 5.2.1.8; FK506-binding protein 1A; FKBP-1A; Immunophilin FKBP12; 12 kDa FKBP; FKBP-12; OTTHUMP00000029978; Rotamase; rotamase; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein, T-cell, 12-kD; peptidyl-prolyl cis-trans isomerase; protein kinase C inhibitor 2
• Gene ID: 2280
• mRNA Refseq: NM_000801
• Protein Refseq: NP_000792
• MIM: 186945
• UniProt ID: P62942
• Chromosome Location: 20p13
• Function: FK506 binding; FK506 binding; SMAD binding; activin binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity; ryanodine-sensitive calcium-release channel activity; signal transducer activity; transforming growth factor beta receptor activity; type I transforming growth factor beta receptor binding