Recombinant Human FICD Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: FICD-1658H
• Product Overview: FICD MS Standard C13 and N15-labeled recombinant protein (NP_009007) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
• Molecular Mass: 51.8 kDa
• AA Sequence: MMLIPMASVMAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: FICD FIC domain containing [ Homo sapiens (human) ]
• Official Symbol: FICD
• Synonyms: FICD; FIC domain containing; adenosine monophosphate-protein transferase FICD; fic S phase protein cell division homolog (E. coli); HIP13; huntingtin interacting protein 13; HYPE; HIP-13; AMPylator FICD; huntingtin yeast partner E; FIC domain-containing protein; huntingtin interactor protein E; Huntingtin interacting protein E; huntingtin-interacting protein E; huntingtin-interacting protein 13; fic S-phase protein cell division homolog; UNQ3041; MGC5623
• Gene ID: 11153
• mRNA Refseq: NM_007076
• Protein Refseq: NP_009007
• UniProt ID: Q9BVA6