Recombinant Mouse Ficd Protein, Myc/DDK-tagged
Cat.No. : | Ficd-3015M |
Product Overview : | Purified recombinant protein of mouse full-length FIC domain containing (Ficd), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates. |
Molecular Mass : | 52.2 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Ficd FIC domain containing [ Mus musculus (house mouse) ] |
Official Symbol | Ficd |
Synonyms | FICD; FIC domain containing; adenosine monophosphate-protein transferase FICD; AMPylator FICD; FIC domain-containing protein; huntingtin interactor protein E; huntingtin interacting protein E; Hype; D5Ertd40e; MGC117722 |
Gene ID | 231630 |
mRNA Refseq | NM_001010825 |
Protein Refseq | NP_001010825 |
UniProt ID | Q8BIX9 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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