Recombinant Human Fibroblast Growth Factor Receptor 4, GST-tagged

• Cat.No.: FGFR4-4844H
• Product Overview: Recombinant human FGFR4 (460-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 460-end a.a.
• Description: FGFR4 is a member of the fibroblast growth factor receptor family which play a role in mitogenesis and differentiation. FGFR4 preferentially binds acidic fibroblast growth factor and is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4 gene expression is up-regulated in doxorubicin-treated, apoptosis-resistant cancer cell clones. Ectopic expression of FGFR4 in cancer cells leads to reduced apoptosis sensitivity on treatment with doxorubicin or cyclophosphamide, whereas knockdown of endogenous FGFR4 expression in breast cancer cell lines has the opposite effect.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 65 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 76 nmol/min/mg
• Purity: > 90 %
• Sequences: 460-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Downstream signaling of activated FGFR; Endocytosis; FGF signaling pathway; FGFR ligand binding and activation; FGFR4 ligand binding and activation; FRS2-mediated cascade; IRS-mediated signaling; Insulin receptor signalling cascade; MAPK signaling pathway; PI-3K cascade; Phospholipase C-mediated cascade; Regulation of actin cytoskeleton; Signaling by FGFR; Signaling by Insulin receptor

Gene Information

• Gene Name: FGFR4 fibroblast growth factor receptor 4 [ Homo sapiens ]
• Official Symbol: FGFR4
• Synonyms: FGFR4; TKF; JTK2; CD334; MGC20292; FGFR-4; fibroblast growth factor receptor 4; OTTHUMP00000161429; OTTHUMP00000161430; OTTHUMP00000223539; OTTHUMP00000223541; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; tyrosine kinase related to fibroblast growth factor receptor; EC 2.7.10.1; CD334 antigen; EC 2.7.10
• Gene ID: 2264
• mRNA Refseq: NM_002011
• Protein Refseq: NP_002002
• MIM: 134935
• UniProt ID: P22455
• Chromosome Location: 5q35.1-qter
• Function: ATP binding; fibroblast growth factor 1 binding; fibroblast growth factor 2 binding; fibroblast growth factor binding; fibroblast growth factor receptor activity; heparin binding; nucleotide binding; protein tyrosine kinase activity; receptor activity