Recombinant Human FGR 293 Cell Lysate

• Cat.No.: FGR-6228HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FGR Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog [ Homo sapiens ]
• Official Symbol: FGR
• Synonyms: FGR; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; SRC2; tyrosine-protein kinase Fgr; c fgr; p55c fgr; p55-c-fgr protein; c-fgr protooncogene; proto-oncogene c-Fgr; c-src-2 proto-oncogene; proto-oncogene tyrosine-protein kinase FGR; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr; FLJ43153; MGC75096
• Gene ID: 2268
• mRNA Refseq: NM_001042729
• Protein Refseq: NP_001036194
• MIM: 164940
• UniProt ID: P09769
• Chromosome Location: 1p36.2-p36.1
• Pathway: Alpha-synuclein signaling, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class I PI3K signaling events, organism-specific biosystem; EPHA forward signaling, organism-specific biosystem; Ephrin B reverse signaling, organism-specific biosystem
• Function: ATP binding; Fc-gamma receptor I complex binding; immunoglobulin receptor binding; immunoglobulin receptor binding; non-membrane spanning protein tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphotyrosine binding; phosphotyrosine binding; protein kinase binding; protein kinase binding; protein tyrosine kinase activity