Recombinant Human FGL2 protein, His-Avi,Flag-tagged

• Cat.No.: FGL2-1585H
• Product Overview: Recombinant Human FGL2 protein(Q14314)(Val205-Pro439), fused with N-terminal His and Avi tag and N-terminal Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: N-His-Avi,N-Flag
• Protein Length: Val205-Pro439
• Tag: N-His-Avi,N-Flag
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FGL2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FGL2 fibrinogen-like 2 [ Homo sapiens ]
• Official Symbol: FGL2
• Synonyms: FGL2; fibrinogen-like 2; fibroleukin; pT49; T49; fibrinogen-like protein 2
• Gene ID: 10875
• mRNA Refseq: NM_006682
• Protein Refseq: NP_006673
• MIM: 605351
• UniProt ID: Q14314