Recombinant Human FGL2 protein, His-Avi&Flag-tagged, Biotinylated

• Cat.No.: FGL2-1371H
• Product Overview: Biotinylated Recombinant Human FGL2 protein(Q14314)(Val205-Pro439), fused with N-terminal His and Avi tag and N-terminal Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: Val205-Pro439
• Tag: N-His-Avi,N-Flag
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Anti-FGL2 Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGL2, His Tag with the EC50 of 0.34μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGL2 fibrinogen-like 2 [ Homo sapiens ]
• Official Symbol: FGL2
• Synonyms: FGL2; fibrinogen-like 2; fibroleukin; pT49; T49; fibrinogen-like protein 2
• Gene ID: 10875
• mRNA Refseq: NM_006682
• Protein Refseq: NP_006673
• MIM: 605351
• UniProt ID: Q14314