Recombinant Human FGFR4 protein, hFc-tagged

• Cat.No.: FGFR4-141H
• Product Overview: Recombinant Human FGFR4 protein(P22455)(Leu22-Asp369), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Leu22-Asp369
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Human Beta Klotho, His Tag with an affinity constant of 13.05 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 80-95 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FGFR4 fibroblast growth factor receptor 4 [ Homo sapiens ]
• Official Symbol: FGFR4
• Synonyms: FGFR4; fibroblast growth factor receptor 4; CD334; JTK2; FGFR-4; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; tyrosine kinase related to fibroblast growth factor receptor; TKF; MGC20292
• Gene ID: 2264
• mRNA Refseq: NM_002011
• Protein Refseq: NP_002002
• MIM: 134935
• UniProt ID: P22455