Recombinant Human FGFR4 Protein, Fc/His-tagged

• Cat.No.: FGFR4-2062H
• Product Overview: Recombinant Human FGFR4/FGF Receptor 4 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu22-Asp369) of human FGFR4/FGF Receptor 4 (Accession #NP_998812.1) fused with an Fc, 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Leu22-Asp369
• Description: Fibroblast growth factor receptor 4 is a protein that in humans is encoded by the FGFR4 gene. FGFR4 has also been designated as CD334 (cluster of differentiation 334). The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FGFR4 fibroblast growth factor receptor 4 [ Homo sapiens ]
• Official Symbol: FGFR4
• Synonyms: FGFR4; fibroblast growth factor receptor 4; CD334; JTK2; FGFR-4; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; tyrosine kinase related to fibroblast growth factor receptor; TKF; MGC20292
• Gene ID: 2264
• mRNA Refseq: NM_002011
• Protein Refseq: NP_002002
• MIM: 134935
• UniProt ID: P22455