Recombinant Human FGFR2 protein, His-tagged

• Cat.No.: FGFR2-5755H
• Product Overview: Recombinant Human FGFR2 protein(P21802-3)(Arg152-Glu378(IIIb)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Arg152-Glu378(IIIb)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FGFR2 beta (IIIb), His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FGFR2 fibroblast growth factor receptor 2 [ Homo sapiens ]
• Official Symbol: FGFR2
• Synonyms: FGFR2; fibroblast growth factor receptor 2; bacteria expressed kinase , BEK, CFD1, craniofacial dysostosis 1 , Jackson Weiss syndrome , JWS, keratinocyte growth factor receptor , KGFR; CD332; CEK3; Crouzon syndrome; ECT1; K SAM; Pfeiffer syndrome; TK14; TK25; FGFR-2; FGF receptor; soluble FGFR4 variant 4; bacteria-expressed kinase; hydroxyaryl-protein kinase; keratinocyte growth factor receptor; BEK fibroblast growth factor receptor; protein tyrosine kinase, receptor like 14; BEK; JWS; CFD1; KGFR; BFR-1; K-SAM; FLJ98662
• Gene ID: 2263
• mRNA Refseq: NM_000141
• Protein Refseq: NP_000132
• MIM: 176943
• UniProt ID: P21802