Recombinant Human FGF7 protein(Cys32-Thr194), His-tagged

• Cat.No.: FGF7-282H
• Product Overview: Recombinant Human KGF (P21781) (Cys32-Thr194) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Cys32-Thr194
• Form: Lyophilized from sterile 50mM Tris, 250mM NaCl, pH 7. 5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 3-20 ng/mL.
• Molecular Mass: The recombinant human KGF consists of 179 amino acids and predicts a molecular mass of 21 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 0.01 EU per μg of the protein.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGF7 fibroblast growth factor 7 [ Homo sapiens ]
• Official Symbol: FGF7
• Synonyms: FGF7; fibroblast growth factor 7; fibroblast growth factor 7 (keratinocyte growth factor); keratinocyte growth factor; KGF; FGF-7; heparin-binding growth factor 7; HBGF-7
• Gene ID: 2252
• mRNA Refseq: NM_002009
• Protein Refseq: NP_002000
• MIM: 148180
• UniProt ID: P21781