Recombinant Human FGF13 protein

• Cat.No.: FGF13-521H
• Product Overview: Recombinant Human FGF13 protein was expressed in Escherichia coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 245
• Description: Human FGF-13 encoded by the FGF13 gene, belongs to the FGF-11 subfamily which has four members FGF-11 to FGF-14. These four members were initially referred to as fibroblast growth factor homologous factors (FHFs) because they have high sequence identity with the other FGFs but did not activate FGF receptors (FGFRs) and were not generally considered members of the FGF family. FGF-13 plays a crucial role in neuron polarization and migration in the cerebral cortex. In murine FGF-13 RNA was detected in developing central nervous system in cells, and was also found throughout the peripheral nervous system.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, pH 8.5, 500 mM NaCl.
• Bio-activity: Test in process.
• Molecular Mass: Approximately 27.6 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids.
• AA Sequence: MAAAIASSLIRQKRQAREREKSNACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSELFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST
• Endotoxin: Less than 1 EU/µg of rHuFGF-13 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: FGF13
• Official Symbol: FGF13
• Synonyms: FGF13; fibroblast growth factor 13; FGF2; FHF2; fibroblast growth factor homologous factor 2; FHF-2; FGF-13
• Gene ID: 2258
• mRNA Refseq: NM_001139498
• Protein Refseq: NP_001132970
• MIM: 300070
• UniProt ID: Q92913