Recombinant Human FETUB protein, His-tagged

• Cat.No.: FETUB-3788H
• Product Overview: Recombinant Human FETUB (Cys16-Pro382) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Cys16-Pro382
• Description: Fetuin-B (FETUB), a second member of the fetuin family in mammals, is also known as Fetuin-like protein IRL685, 16G2 and Gugu, which is expressed in liver and testis and contains 2 cystatin fetuin-B-type domains. FETUB is a protease inhibitor required for egg fertilization. FETUB is also required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). As a liver-derived plasma protein, FETUB is essential for fertilization.
• Predicted N Terminal: Cys16
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 42.4 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FETUB
• Official Symbol: FETUB
• Synonyms: FETUB; fetuin B; fetuin-B; fetuin-like protein IRL685; 16G2; Gugu; IRL685
• Gene ID: 26998
• mRNA Refseq: NM_014375
• Protein Refseq: NP_055190
• MIM: 605954
• UniProt ID: Q9UGM5