Recombinant Human FEN1 Protein, His-tagged

• Cat.No.: FEN1-151H
• Product Overview: Active recombinant Human full length FEN1 Protein with a C-terminal His tag was protein expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-380
• Description: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
• Molecular Mass: 43.8 kDa
• Purity: ≥85% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme to produce 1 pmol of FLAP per minute at 25 centigrade in 50 mM Tris, pH8.0, 10 mM MgCl2, 1 mM DTT, and 0.01% Tween20.
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Tris, pH 8.0, containing 1 mM DTT and 10% glycerol

Gene Information

• Gene Name: FEN1 flap structure-specific endonuclease 1 [ Homo sapiens (human) ]
• Official Symbol: FEN1
• Synonyms: FEN1; flap structure-specific endonuclease 1; MF1; RAD2; FEN-1; flap endonuclease 1; DNase IV; maturation factor-1; EC 3.1.26.4
• Gene ID: 2237
• mRNA Refseq: NM_004111
• Protein Refseq: NP_004102
• MIM: 600393
• UniProt ID: P39748