Recombinant Human FBL Protein, His-tagged

• Cat.No.: FBL-85H
• Product Overview: Recombinant Human FBL(Gly37~Ala275) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gly37~Ala275
• Description: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 28.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: FBL fibrillarin [ Homo sapiens ]
• Official Symbol: FBL
• Synonyms: FBL; fibrillarin; rRNA 2-O-methyltransferase fibrillarin; FIB; FLRN; RNU3IP1; 34-kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
• Gene ID: 2091
• mRNA Refseq: NM_001436
• Protein Refseq: NP_001427
• MIM: 134795
• UniProt ID: P22087