Recombinant Human FAS protein, His-tagged

• Cat.No.: FAS-614H
• Product Overview: Recombinant Human FAS (P25445) aa. (Gln26~Asn173) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gln26~Asn173
• Description: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.2kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: FAS Fas cell surface death receptor [ Homo sapiens (human) ]
• Official Symbol: FAS
• Synonyms: FAS; Fas cell surface death receptor; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; tumor necrosis factor receptor superfamily member 6; APO-1 cell surface antigen; CD95 antigen; FASLG receptor; Fas (TNF receptor superfamily, member 6); Fas AMA; TNF receptor superfamily member 6; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; mutant tumor necrosis receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
• Gene ID: 355
• mRNA Refseq: NM_000043.5
• Protein Refseq: NP_000034.1
• UniProt ID: P25445