Recombinant Human FAP protein, His-Avi-tagged, Biotinylated

• Cat.No.: FAP-884HB
• Product Overview: Biotinylated Recombinant Human FAP protein(Q12884)(Leu26-Asp760), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu26-Asp760
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tirs, 150mM NaCl, 200mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FAP fibroblast activation protein, alpha [ Homo sapiens ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451
• MIM: 600403
• UniProt ID: Q12884