Recombinant Human EZH2 Complex protein, His/Flag-tagged

• Cat.No.: EZH2-164H
• Product Overview: Mutant version of EZH2 5-member complex, but with a Glu-to-Lys mutation on a.a. 745 of the EZH2 protein.* Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; E745K) with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His&Flag
• ProteinLength: 2–end (all components)
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
• Molecular Mass: 325 kDa (complex)
• AA Sequence: MHHHHHHGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVH ILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDG TFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKF PSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKY DCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNN SSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAE ASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKK DGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDL CLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYD KYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADA LKYVGIKREMEIP
• Purity: ≥ 81%
• Applications: Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
• Storage: >6 months at -70 centigrade. Avoid freeze/thaw cycles
• Concentration: 0.38 mg/ml

Gene Information

• Gene Name: EZH2 enhancer of zeste homolog 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: EZH2
• Synonyms: EZH2; enhancer of zeste homolog 2 (Drosophila); enhancer of zeste (Drosophila) homolog 2; histone-lysine N-methyltransferase EZH2; ENX 1; EZH1; KMT6; KMT6A; lysine N-methyltransferase 6; ENX1; WVS2; ENX-1; MGC9169
• Gene ID: 2146
• mRNA Refseq: NM_004456
• Protein Refseq: NP_004447
• MIM: 601573
• UniProt ID: Q15910
• Chromosome Location: 7q35-q36
• Function: DNA binding; histone methyltransferase activity; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transferase activity