Recombinant Human EZH1 protein, His-tagged

• Cat.No.: EZH1-1742H
• Product Overview: Recombinant Human EZH1 aa. (Pro559~Leu747) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro559~Leu747
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mMNaHCO3 , 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 8.1

Gene Information

• Gene Name: EZH1 enhancer of zeste 1 polycomb repressive complex 2 subunit [ Homo sapiens (human) ]
• Official Symbol: EZH1
• Synonyms: EZH1; enhancer of zeste 1 polycomb repressive complex 2 subunit; KMT6B; histone-lysine N-methyltransferase EZH1; ENX-2; enhancer of zeste homolog 1
• Gene ID: 2145
• mRNA Refseq: NM_001321079.1
• Protein Refseq: NP_001308008.1
• UniProt ID: Q92800